Megazyme K-XylX6-2V: Xylanase Assay Kit (XylX6 Method)
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Product code: K-XylX6-2V
[/vc_column_text][vc_column_text]The XylX6 assay reagent for the measurement of endo-xylanase (endo-1,4-β-xylanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-45-glucosyl-xylopentaoside and 2) β-xylosidase. The ketone blocking group prevents any hydrolytic action by the β-xylosidase or other exo-acting glycosidases on the XylX6 substrate. Incubation with an endo-xylanase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-xylosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of XylX6 by the endo-xylanase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).[/vc_column_text][vc_empty_space height=”20px”][vc_row_inner][vc_column_inner width=”1/2″][dt_button link=”https://wa.me/6281380382133″ target_blank=”true” button_alignment=”center” animation=”bounce” size=”big” icon=”JTNDaSUyMGNsYXNzJTNEJTIyZmElMjBmYS1waG9uZSUyMiUzRSUzQyUyRmklM0U=”]Call Us[/dt_button][/vc_column_inner][vc_column_inner width=”1/2″][dt_button link=”https://tsl.mwd.my.id/contact-us/” target_blank=”true” button_alignment=”center” animation=”bounce” size=”big” icon=”JTNDaSUyMGNsYXNzJTNEJTIyZmElMjBmYS1waG9uZSUyMiUzRSUzQyUyRmklM0U=”]Email Us[/dt_button][/vc_column_inner][/vc_row_inner][vc_empty_space height=”10px”][/vc_column][/vc_row][vc_row][vc_column][vc_empty_space][/vc_column][/vc_row][vc_row type=”4″][vc_column width=”1/2″][vc_empty_space height=”60px”][dt_vc_list]
| Content: | 200 assays (manual) / 400 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | endo-1,4-β-Xylanase |
| Assay Format: | Spectrophotometer, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400, 405 |
| Signal Response: | Increase |
| Limit of Detection: | 5.3 x 10-4 U/mL |
| Reproducibility (%): | ~ 3% |
| Total Assay Time: | 10 min |
| Application examples: | Fermentation broths, industrial enzyme preparations, animal feed, biofuels research, barley malt analysis. |
| Method recognition: | Novel method |
[/dt_vc_list][vc_row_inner][vc_column_inner][/vc_column_inner][/vc_row_inner][/vc_column][vc_column width=”1/2″][vc_empty_space height=”60px”][vc_column_text][/vc_column_text][vc_row_inner][vc_column_inner width=”1/2″][vc_single_image image=”9156″ img_size=”full” alignment=”center” onclick=”zoom”][/vc_column_inner][vc_column_inner width=”1/2″][vc_column_text]Note: Standard curves relating the absorbance obtained using the XylX6 assay to endo-xylanase activity on the native substrates, wheat arabinoxylan and beechwood xylan, are provided in the Supporting Information file below.[/vc_column_text][/vc_column_inner][/vc_row_inner][dt_vc_list]
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