Megazyme K-MRGLUC: D-Glucose Assay Kit Megaplex Red
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Product code: K-MRGLUC
[/vc_column_text][vc_column_text]The D-Glucose Assay Kit (Megaplex Red) provides a simple robust method for the measurement of D-glucose. This kit utilises a highly sensitive Megaplex Red probe which when coupled to horseradish peroxidase (HRP) and glucose oxidase (GOX) enzymatic reactions, allows for the measurement of D-glucose in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation between 530-560 nm and emission at ~ 590 nm* or colourimetrically at 570 nm.
*The excitation and emission maxima of Megaplex Red are 570 nm and 585 nm respectively.[/vc_column_text][vc_empty_space height=”20px”][vc_row_inner][vc_column_inner width=”1/2″][dt_button link=”https://wa.me/6281380382133″ target_blank=”true” button_alignment=”center” animation=”bounce” size=”big” icon=”JTNDaSUyMGNsYXNzJTNEJTIyZmElMjBmYS1waG9uZSUyMiUzRSUzQyUyRmklM0U=”]Call Us[/dt_button][/vc_column_inner][vc_column_inner width=”1/2″][dt_button link=”https://tsl.mwd.my.id/contact-us/” target_blank=”true” button_alignment=”center” animation=”bounce” size=”big” icon=”JTNDaSUyMGNsYXNzJTNEJTIyZmElMjBmYS1lbnZlbG9wZS1vJTIyJTNFJTNDJTJGaSUzRQ==”]Email Us[/dt_button][/vc_column_inner][/vc_row_inner][vc_empty_space height=”10px”][/vc_column][/vc_row][vc_row][vc_column][vc_empty_space][/vc_column][/vc_row][vc_row type=”4″][vc_column width=”1/2″][vc_empty_space height=”60px”][dt_vc_list]
| Content: | 1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 6 months under recommended storage conditions |
| Analyte: | D-Glucose |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance, Fluorescence |
| Wavelength (nm): | (Fluorescence) 530-560 (ex), ~ 590 (em) (Absorbance) 570 |
| Signal Response: | Increase |
| Linear Range: | (Fluorescence) 0.009-0.18 μg of D-glucose per assay (0.5-10 μM in-assay) (Absorbance) 0.9-9.0 μg of D-glucose per assay (5-50 μM in-assay) |
| Limit of Detection: | (Fluorescence) 0.09 ug/mL (Absorbance) 0.25 ug/mL |
| Reproducibility (%): | (Fluorescence) ~ 6% (Absorbance) ~ 7% |
| Reaction Time (min): | (Fluorescence) 30 min (Absorbance) 30 min |
| Application examples: | Food and beverage samples such as wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery products, candies, fruit and vegetables. Other samples such as tobacco, cosmetics, pharmaceuticals (e.g. infusions), feed, paper (and cardboard) and other materials (e.g. biological cultures, samples, etc.). |
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