Half Fraser (Demi-Fraser) Broth (ISO)

[vc_row][vc_column width=”1/2″][vc_single_image image=”7154″ img_size=”full” alignment=”center” style=”vc_box_shadow” onclick=”custom_link” img_link_target=”_blank” css_animation=”fadeIn” link=”https://www.neogen.com/”][/vc_column][vc_column width=”1/2″][ultimate_heading source=”post_title” heading_tag=”h3″ alignment=”left”][/ultimate_heading][vc_empty_space][vc_column_text]

• Item No. NCM0001A – 500 g
• Item No. NCM0001B – 5 kg
• Item No. NCM0001C – 10 kg
• Item No. NCM0001D – 25 kg
• Item No. NCM0001E – 50 kg

[/vc_column_text][vc_empty_space height=”20px”][vc_row_inner][vc_column_inner width=”1/3″][dt_button link=”https://wa.me/6281380382133″ target_blank=”true” button_alignment=”center” animation=”bounce” size=”big” bg_color_style=”custom” bg_color=”#006633″ icon=”JTNDaSUyMGNsYXNzJTNEJTIyZmElMjBmYS1waG9uZSUyMiUzRSUzQyUyRmklM0U=”]Call us[/dt_button][/vc_column_inner][vc_column_inner width=”1/3″][dt_button link=”https://tsl.mwd.my.id/contact-us/” button_alignment=”center” animation=”bounce” size=”big” bg_color_style=”custom” bg_color=”#006633″ icon=”JTNDaSUyMGNsYXNzJTNEJTIyZmElMjBmYS1lbnZlbG9wZS1vJTIyJTNFJTNDJTJGaSUzRQ==”]Email us[/dt_button][/vc_column_inner][vc_column_inner width=”1/3″][dt_button link=”https://tsl.mwd.my.id/brochure-request-form/” button_alignment=”center” animation=”bounce” size=”big” bg_color_style=”custom” bg_color=”#006633″ icon=”JTNDaSUyMGNsYXNzJTNEJTIyZmElMjBmYS1kb3dubG9hZCUyMiUzRSUzQyUyRmklM0U=”]Download[/dt_button][/vc_column_inner][/vc_row_inner][vc_empty_space height=”10px”][/vc_column][/vc_row][vc_row][vc_column][vc_empty_space][/vc_column][/vc_row][vc_row type=”4″][vc_column][vc_empty_space height=”60px”][dt_vc_list]Half Fraser (Demi-Fraser) Broth is used with ferric ammonium citrate for the selective enrichment of Listeria species and is not intended for use in the diagnosis of disease or other conditions in humans. Primary enrichment broth for the isolation of Listeria spp. formulated according to ISO 11290. The selective components acriflavine and nalidixic acid are blended into the base powder and the ferric ammonium citrate is added to the tempered broth after sterilization. Half Fraser (Demi-Fraser) Broth, NCM0001 was developed to give improved results for the isolation of Listeria spp. with both traditional and ELISA methods. As with the sister product, Fraser Broth, NCM0050 the selective components acriflavine and nalidixic acid are blended into the base powder and the Ferric Ammonium Citrate is added to the tempered broth after autoclaving. This format has been shown to give improved selectivity with pure cultures and food samples. Furthermore, more stable ELISA results are seen, resulting in fewer false positive results when the ferric ammonium citrate is omitted from the complete media. Developed as a modification of UVM medium (NCM0012) and made according to ISO 11290, Fraser Broth is a secondary enrichment broth for the isolation of Listeria spp. and is similar to Palcam Broth (NCM0049) in that it contains esculin to indicate the presence of a potential Listeria isolate. It also contains lithium chloride in an attempt to suppress the growth of Enterococci in the medium (as does Palcam). Listeria spp. hydrolyze the esculin to form esculetin, which reacts with the ferric ammonium citrate in resulting in a black precipitate and a visible positive reaction. However, Enterococci can also perform this reaction so further plating is required onto an isolation medium such as Harlequin® Listeria Chromogenic Agar (NCM1004).[/dt_vc_list][vc_empty_space height=”60px”][/vc_column][/vc_row][vc_row][vc_column][vc_empty_space height=”60px”][/vc_column][/vc_row][vc_row][vc_column width=”1/2″][vc_column_text]

Specifications
Brand	Neogen® Culture Media
Analyte	Listeria spp.
Platform	Dehydrated Culture Media
Mass	50 kg
Formula	Formula Liter Enzymatic Digest of Animal Tissues 5.0 g/L Enzymatic Digest of Casein 5.0 g/L Meat Extract 5.0 g/L Yeast Extract 5.0 g/L Esculin 1.0 g/L Disodium Hydrogen Phosphate 9.6 g/L* Potassium Dihydrogen Phosphate 1.35 g/L Sodium Chloride 20.0 g/L Lithium Chloride 3.0 g/L Acriflavine 0.0125 g/L Nalidixic Acid 0.01 g/L *Equivalent to Disodium Hydrogen Phosphate Dihydrate 12.0 g/L
Grams per Liter	55.00
Liters per 500 g	9.00
Preparation	Dissolve 55 grams of the medium in one liter of purified water. Mix thoroughly. Autoclave at 121°C for 15 minutes 4. Cool to 45-50°C. Aseptically add 2 vials of NCM4009-0.5* Ferric Ammonium Citrate Supplement. *Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.
Previous Item Number	Product was previously known as Acumedia Product: 7656 Demi-Fraser Broth Base; and LabM Product: LAB211 Half Fraser Broth PLUS
Package Dimensions	18.00 in x 18.00 in x 30.00 in
Package Weight	125.00 lb

[/vc_column_text][/vc_column][vc_column width=”1/2″][dt_vc_list]

• TECHNICAL SPECIFICATION SHEET
• SAFETY DATA SHEET (SDS)

[/dt_vc_list][/vc_column][/vc_row][vc_row type=”4″][vc_column][vc_empty_space][vc_column_text]For more information, please Contact us

or visit NEOGEN’s website[/vc_column_text][vc_empty_space height=”10px”][/vc_column][/vc_row]