Megazyme E-SUCR: Sucrase (from yeast)
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Product code: E-SUCR
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High purity Sucrase (from yeast) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. Sucrase (E-SUCR) and Fructanase (E-FRMXLQ or E-FRMXPD) are used in the measurement of fructan in foods according to Chinese GB Standard 5009.255-2016 and in the AOAC method 2016.06.
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| Enzyme Activity: | Sucrase/Invertase |
| EC Number: | 3.2.1.20 |
| CAZy Family: | GH13 |
| CAS Number: | 9001-42-7 |
| Synonyms: | alpha-glucosidase; alpha-D-glucoside glucohydrolase |
| Source: | Yeast |
| Molecular Weight: | 62,000 |
| Expression: | Purified from Yeast |
| Specificity: | Hydrolysis of terminal, non-reducing (1,4)-linked α-D-glucose residues with release of D-glucose. |
| Specific Activity: | ~ 20 U/mg (30oC, pH 6.8 on sucrose) |
| Unit Definition: | One Unit of sucrase activity is defined as the amount of enzyme required to release one µmole of glucose per minute from sucrose (10 mM) in sodium maleate buffer (100 mM), pH 6.8 at 30oC. |
| Temperature Optima: | 30oC |
| pH Optima: | 6.8 |
| Application examples: | Applications for the removal of sucrose in various analytical procedures in the cereals, food and feeds, fermentation and beverage industries. |
| Method recognition: | AOAC Method 2016.06 and GB Standard 5009.255-2016 |
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