Palcam Agar Base

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Used together with Palcam PAC supplement (NCM4041-0.5) as a selective and differential medium for the detection and isolation of Listeria monocytogenes from food and environmental samples.

Palcam Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

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Palcam Agar was developed by Van Netten et al in 1989 as an improved selective differential medium for the isolation of Listeria monocytogenes from food and environmental specimens. Improved selectivity is achieved by the combination of antibiotic supplements and microaerobic incubation. The double indicator system of esculin hydrolysis and mannitol fermentation aids differentiation of Listeria spp from enterococci and staphylococci which can be confused with Listeria spp on other types of culture media. Palcam Agar is listed within Annex E of ISO 11290-1:2017 as a suitable second selective agar.

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Specifications
Brand	Neogen® Culture Media
Analyte	Listeria monocytogenes
Platform	Dehydrated Culture Media
Mass	500 g
Formula	Formula Liter Columbia Peptone Mix 23.0 g/L Sodium Chloride 5.0 g/L Corn Starch 1.0 g/L Yeast Extract 3.0 g/L Glucose 0.5 g/L Mannitol 10.0 g/L Esculin 0.8 g/L Lithium Chloride 15.0 g/L Ferric Ammonium citrate 0.5 g/L Phenol Red 0.08 g/L Agar 12.0 g/L
Grams per Liter	71.00
Liters per 500 g	7.00
Plates per 500 g	380.00
Preparation	Suspend 71 grams of the medium in one liter of purified water. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Autoclave at 121°C for 15 minutes. Cool to 45-50°C 5. Aseptically add 2 vials of NCM4041-0.5* Palcam PAC Supplement, each reconstituted using 5 mL sterile deionized/RO water. Mix well. *Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.
Previous Item Number	Product was previously known as Acumedia Product: 7669 PALCAM Agar; and LabM Product: LAB148 PALCAM Agar Base
Package Weight	1.35 lb

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• TECHNICAL SPECIFICATION SHEET
• SAFETY DATA SHEET (SDS)

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